P-6002-10PK，P-6002-2PK脂质研究工具解析

P-6002-10PK，P-6002-2PK脂质研究工具——艾美捷膜脂试纸条。一起来看看，如何快速筛选和鉴定，目标蛋白与哪类膜脂会结合并产生？

艾美捷 P-6002-10PK，P-6002-2PK Membrane Lipid Strips是一种2 * 6 cm的疏水膜试纸条，包含了细胞膜中常见的15种具有不同生物功能的重要脂质，每种脂类含量均为100 pmol。通过一个简单的蛋白质-脂质覆盖实验（PLO）来确定脂质-蛋白质之间的相互作用。这为研究人员提供了一种方便的方法来确定他们感兴趣的蛋白质是否与一种或多个结合的脂质相互作用。

艾美捷 P-6002-10PK，P-6002-2PK已发表高分认证文章——Cell, Nature（见文末）斑点分布如下：

艾美捷 P-6002-10PK，P-6002-2PK相关脂类研究：

脂类英文 中文

Glyceryl tripalmitate or Triglyceride (GT) 甘油三酯（GT）

Diacylglycerol (DAG, L-0016) 甘油二脂(DAG, L-0016)

Phosphatidic Acid (PA, L-4116) 磷脂酸 (PA, L-4116)

Phosphatidylserine (PS, L-3116) 磷脂酰丝氨酸 (PS, L-3116)

Phosphatidylethanolamine (PE, L-2116) 磷脂酰乙丨醇丨胺 (PE, L-2116)

Phosphatidylcholine (PC, L-1116) 磷脂酰胆碱 (PC, L-1116)

Phosphatidylglycerol (PG, L-5116) 磷脂酰甘油 (PG, L-5116)

Cardiolipin (CL, L-C160) 心磷脂 (CL, L-C160)

Phosphatidylinositol (PI P-0016) 磷脂酰肌醇 (PI P-0016)

PtdIns(4)P (P-4016) 磷脂酰肌醇(4)-磷酸 (P-4016)

PtdIns(4,5)P2 (P-4516) 磷脂酰肌醇(4,5)-二磷酸 (P-4516)

PtdIns(3,4,5)P3 (P-3916) 磷脂酰肌醇(3,4,5)-三磷酸酯 (P-3916)

Cholesterol (L-6012) 胆固醇 (L-6012)

Sphingomyelin (SM) 鞘磷脂 (SM)

Sulfatides 硫酸脑苷脂

艾美捷 P-6002-10PK，P-6002-2PK Nature/Cell文章鉴赏：

①Nature文章：Liu, Xing, et al. "Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores." Nature 535.7610 (2016): 153-158.

② Cell文章：Liao, Ya-Cheng, et al. "RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether." Cell 179.1 (2019): 147-164.

Nature/Cell 已发表文章（部分）：

Lee, Jaewoong, et al. "IFITM3 functions as a PIP3 scaffold to amplify PI3K signalling in B cells." Nature 588.7838 (2020): 491-497.

Uzureau, Sophie, et al. "APOL1 C-terminal variants may trigger kidney disease through interference with APOL3 control of actomyosin." Cell reports30.11 (2020): 3821-3836.

Suarez, Catherine, et al. "A lipid-binding protein mediates rhoptry discharge and invasion in Plasmodium falciparum and Toxoplasma gondii parasites." Nature communications10.1(2019): 1-14.

Liao, Ya-Cheng, et al. "RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether." Cell 179.1(2019): 147-164.