SouthernBiotech 丨HRP标记Avidin亲和素的应用参数

HRP标记Avidin亲和素中性粒抗生物素：

Cat. No. Format Size

7200-01 Purified (UNLB) 5.0 mg

7200-02 Fluorescein (FITC) 1.0 mg

7200-03 Rhodamine (TRITC) 1.0 mg

7200-04 Alkaline Phosphatase (AP) 1.0 mL

7200-05 Horseradish Peroxidase (HRP) 1.0 mL

7200-06 β-galactosidase (BGAL) 1.0 mL

7200-07 Texas Red (TXRD) 1.0 mg

7200-09 R-phycoerythrin (PE) 0.5 mg

7200-11 Allophycocyanin (APC) 0.1 mg

7200-12 Cyanine 3 (CY3) 0.1 mg

7200-13 Spectral Red® (SPRD) 0.1 mg

7200-15 Cyanine 5 (CY5) 1.0 mg

艾美捷 SouthernBiotech  HRP标记Avidin亲和素描述：

特异性：生物素

来源：中和（pI=6.3±0.3），从蛋清中纯化去糖基化抗生物素

艾美捷 SouthernBiotech  HRP标记Avidin亲和素应用：

经过质量测试的应用包括——

ELISA

FLISA

FC

其他参考应用包括——

ELISPOT

IHC-FS

IHC-PS

ICC

WB

Microarray

艾美捷 SouthernBiotech  HRP标记Avidin亲和素工作稀释液：

流式细胞术：

FITC和CY3共轭物≤ 1µg/106个细胞

PE、APC、SPRD和CY5共轭物≤ 0.2µg/106个细胞

对于流式细胞术，建议使用这些试剂的最终体积为100µL

FLISA：

FITC、TRITC、TXRD和CY3共轭物1:100–1:400

PE、APC和CY5共轭物≤ 1微克/毫升

ELISA：

AP共轭1:500–1:2000

HRP共轭1:4000–1:8000

BGAL共轭1:500

SouthernBiotech HRP标记Avidin亲和素部分参考文献：

1. Brown MP, Topham DJ, Sangster MY, Zhao J, Flynn KJ, Surman SL, et al. Thymic lymphoproliferative disease after successful correction of CD40 ligand deficiency by gene transfer in

mice. Nat Med. 1998;4:1253-60. (ELISA)

2. Reinagel ML, Taylor RP. Transfer of immune complexes from erythrocyte CR1 to mouse macrophages. J Immunol. 2000;164:1977-85. (ELISA, FC)

3. Hodge LM, Marinaro M, Jones HP, McGhee JR, Kiyono H, Simecka JW. Immunoglobulin A (IgA) responses and IgE-associated inflammation along the respiratory tract after mucosal

but not systemic immunization. Infect Immun. 2001;69:2328-38. (ELISA, ELISPOT)

来源：https://www.amyjet.com/products/7200-05.shtml