专用荧光检测器丨艾美捷游离巯基检测试剂盒

The detection and measurement of free thiols (i.e., free cysteine, glutathione, and cysteine residues on proteins) is one of the essential tasks for investigating biological processes and events in many biological systems. Cayman’s Thiol Detection Assay Kit provides a simple, reproducible, and sensitive tool for assaying free thiol content in samples (i.e., plasma, serum, tissue homogenates, cell lysates, and urine). The assay utilizes a proprietary fluorometric detector that reacts with thiol groups to emit a strong fluorescent signal which can be detected using excitation wavelengths between 380-390 nm and emission wavelengths between 510-520 nm.

游离硫醇（即蛋白质上的游离半胱氨酸、谷胱甘肽和半胱氨酸残基）的检测和测量是研究许多生物系统中的生物过程和事件的基本任务之一。Cayman硫醇检测分析试剂盒提供了一种简单、可重复和灵敏的工具，用于分析样品（即血浆、血清、组织匀浆、细胞裂解物和尿液）中的游离硫醇含量。该测定使用专有的荧光检测器，该检测器与硫醇基反应以发出强烈的荧光信号，该荧光信号可以使用380-390nm之间的激发波长和510-520nm之间的发射波长来检测。

艾美捷游离巯基检测试剂盒基本参数：

中文名称：硫醇分析试剂盒-96次分析

英文名字：Thiol Detection Assay Kit

编号：700340-96

规格：96wells

背景资料：检测和定量游离硫醇是研究多种生物物种生物进程和事件的重要指标.

相关描述：硫醇分析试剂盒提供了一个简便方法检测血液、尿液、组织匀浆和细胞裂解液中游离的硫醇.利用一个砖利的荧光指示剂作用于硫醇时将会产生强荧光信号，以对应硫醇的含量.

特点：该试剂盒通过荧光法来检测硫醇，激发波长为380-390nm，发射波长为510-520nm.

保存建议：将其置于-20°C下保存.

艾美捷游离巯基检测试剂盒特点：

1.测量血浆、血清、尿液、细胞裂解物和组织匀浆中的游离硫醇含量

2.测定40份样品，一式两份

3.测定范围：15 nM-1µM

4.基于平板的荧光测量（ex 380-390 nm，em 510-520 nm）

5.包括谷胱甘肽和半胱氨酸标准

游离巯基检测试剂盒文献参考：

1.Wlodek, L. Beneficial and harmful effects of thiols. Polish Journal of Pharmacology 54, 215-223 (2002).

2.Lash, L.H. and Jones, D.P. Distribution of oxidized and reduced forms of glutathione and cysteine in rat plasma. Arch. Biochem. Biophys. 240, 583-592 (1985).

3.Wendel, A. and Cikryt, P. The level and half-life of glutathione in human plasma. FEBS Lett. 120, 209-211 (1980).

4.Dickenson, D., Forman, H. Cellular glutathione and thiols metabolism.

Biochemical Pharmacology 64, 1019-1026 (2002).

来源：https://www.amyjet.com/products/700340-96.shtml