艾美捷12(S)-HETE-ELISA试剂盒，比色竞争性测定

The 12(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded reagents that reduce error.

Easy-to-use color-coded reagents and pre-coated plates reduce errors and save time in the lab

High throughput format, obtain results from up to 39 samples in duplicate in just 5 hours

Highly sensitive assay, measure as little as 146 pg/ml

Reliable and reproducible results day-after-day and lot-after-lot

Sensitivity: 146 pg/ml (range 195 - 50,000 pg/ml)

Assay Time: 5 hours

Applications: ELISA, Colorimetric detection

Application Notes: For the quantitative determination of 12(S)-HETE in culture supernatants and plasma from any species. Cited sample types include serum, renal interstitial fluid and tissue.

Wavelength: 405 nm

Species reactivity: Species independent

Use/Stability: Store all components at +4°C, except standard and conjugate at -20°C.

Shipping: Shipped on Blue Ice

Contents: GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

12（S）-HETE对多形核白细胞和血管平滑肌细胞具有趋化性和化学动力学。它也作为血管紧张素II诱导的醛固酮产生的第二信使。

12(S)-HETE-ELISA试剂盒是一种比色竞争性酶免疫测定试剂盒，5小时内即可获得结果。在405 nm处读取吸光度。使用易于使用的液体色码试剂可获得快速、准确和可靠的结果，从而减少误差。

艾美捷12(S)-HETE-ELISA试剂盒——灵敏的ELISA试剂盒能够检测来自任何物种的培养上清液和血浆中的12（S）-HETE。

12(S)-HETE-ELISA试剂盒化学性质：

替代名称：12（S）-氢过氧四烯酸二十碳四烯酸

灵敏度：146 pg/ml（范围195-50000 pg/ml）

化验时间：5小时

应用：ELISA，比色检测

应用说明：用于定量测定任何物种培养上清液和血浆中的12（S）-HETE。引用的样本类型包括血清、肾间质液和组织。

波长：405 nm

物种反应性：物种无关

使用/稳定性：在+4°C下储存所有成分，-20°C下标准和共轭物除外。

运输：蓝冰运输

内容：GxR IgG微量滴定板、缀合物、抗体、测定缓冲液、洗涤缓冲液浓缩液、标准品、pNpp底物、停止溶液

12(S)-HETE-ELISA试剂盒艾美捷特点：

1.易于使用的色码试剂和预涂板减少了错误并节省了实验室时间

2.高通量格式，仅需5小时即可获得多达39份样本的结果，一式两份

3.高灵敏度测定，测量值低至146 pg/ml

4.日复一日、一批又一批可靠且可重复的结果

12(S)-HETE-ELISA试剂盒文献参考：

12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016), Application(s): Measurement of HETE levels in rats, Abstract; Full Text

Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching: C.H. Nguyen, et al.; Br. J. Cancer 115, 364 (2016), Application(s): 12(S)-HETE assay, rabbit, Abstract; Full Text

12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res. 56, 502 (2015), Application(s): ELISA, Abstract; Full Text

Lobatin b inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation: I. Kiss, et al.; Cancer Lett. 356, 994 (2015), Application(s): ELISA using MCF7 cell culture supernatants,

来源：https://www.amyjet.com/products/ADI-900-050.shtml