艾美捷一氧化氮(总)检测试剂盒应用说明

一氧化氮（总）检测试剂盒（ADI-917-020）基于硝酸还原酶将硝酸盐酶促转化为亚硝酸盐，然后进行格里斯反应以形成有色偶氮染料产物。通过测量540-570nm处的吸收进行定量。每孔仅需 0.050 ml 稀释样品，可节省宝贵的样品。

艾美捷一氧化氮(总)检测试剂盒基于硝酸盐还原酶将硝酸盐酶转化为亚硝酸盐，然后通过Griess反应形成有色偶氮染料产物。通过测定540 - 570 nm处的吸光度进行定量。每孔仅需0.050ml稀释样品，节省了宝贵的样品。灵敏、高效、简便的总一氧化氮检测方法，只需40应用。

一氧化氮(总)检测试剂盒基参：

敏感性： 0.625 微摩尔/升（范围 3.125 - 100 微摩尔/升）

应用： 比色检测

应用说明： 用于定量测定培养物上清液、血浆、血清、唾液和尿液中任何物种的总一氧化氮。引用的样本类型包括组织（参考文献）。

物种反应性： 物种独立

使用/稳定性： 将所有组分储存在 4°C，硝酸盐还原酶除外，在 -20°C 下储存。

航运： 在蓝冰上运输

内容： 微量滴定板，硝酸盐还原酶，硝酸盐还原酶储存缓冲液，NADH，硝酸盐标准品，反应缓冲液浓缩物，格里斯试剂I，格里斯试剂II

一氧化氮(总)检测试剂盒（ADI-917-020）特点：

Efficient - only 50 µl diluted sample needed, saving your precious sample

Sensitive - detect as little as 0.625 µmol/L

Trusted - widely cited in peer-reviewed publications

Fast - results from up to 80 samples in duplicate in just 40 minutes

一氧化氮(总)检测试剂盒部分文献参考：

1-Oleyl-lysophosphatidic acid (LPA) promotes polarization of BV-2 and primary murine microglia towards an M1-like phenotype: I. Plastira, et al.; J. Neuroinflammation 13, 205 (2016), Application(s): iNOS activity assessment (through measuring accumulated total nitrate levels) primary microglia and BV-2 cell supernatant, Abstract;

Neuronal NOS Activates Spinal NADPH Oxidase 2 Contributing to Central Sigma-1 Receptor-Induced Pain Hypersensitivity in Mice: S.R. Choi, et al.; Biol. Pharm. Bull. 39, 1922 (2016);

Peroxiredoxin 6 expression is inversely correlated with nuclear factor-κB activation during Clonorchis sinensis infestation: J.H. Pak, et al.; Free Radic. Biol. Med. 99, 273 (2016), Application(s): Measuring nitrite/nitrate concentration in culture medium;

来源：https://www.amyjet.com/products/ADI-917-020.shtml