中英文说明书丨艾美捷 RmesFect CRISPR转染试剂

RmesFect™ CRISPR transfection Reagent based on the TEE-technology is specifically designed for mRNA/gRNA transfection with high efficiency and low toxicity. RmesFect™ is efficient in a large variety of cells.

Benefits:

Highly efficient: achieve RNAs delivery in all cells

Ready-to-use: no need for additional buffer

Low nucleic acid amount : minimized toxicity

Protects RNAs against degradation

Compatible with any culture medium

Serum compatible : Medium changed not required

mRNA transfection provides several advantages over plasmid DNA delivery :

No need for nuclear uptake. mRNA translation into proteins occurs in the cytoplasm.

Faster protein expression than DNA transfection

No genomic integration

Protein expression in a total promoter-independent manner

Additional products for CRISPR Cas9 experiments:

PolyMag CRISPR for Genome editing using expression plasmids

ProDeliverIN CRISPR for Cas9 protein delivery

ViroMag CRISPR to enhance transduction efficiency of CRISPR/Cas9 viruses

基于TEE技术的艾美捷 RmesFect™ CRISPR转染试剂专为mRNA/gRNA转染而设计，具有高效率和低毒性的特点。RmesFect™在多种细胞中都是有效的。

OZ Biosciences RmesFect CRISPR转染试剂特点：

高效：在所有细胞中实现RNA递送

即用型：无需额外的缓冲液

低核酸量：毒性最小化

保护 RNA 免受降解

与任何培养基兼容

血清兼容：无需更换培养基

图 1：根据标准方案，使用 RmesFect CRISPR 转染编码 GFP 和 RFP（各 24.0μg）的 mRNA 转染 DC25 细胞。转染后24h在显微镜荧光下分析图像。

与质粒DNA递送相比，mRNA转染具有以下优势：

无需核吸收。mRNA翻译成蛋白质发生在细胞质中。

蛋白表达速度快于DNA转染

无基因组整合

以完全启动子非依赖的方式表达蛋白质

用于CRISPR Cas9实验的其他产品：

PolyMag CRISPR用于使用表达质粒进行基因组编辑

ProDeliverIN CRISPR用于 Cas9 蛋白递送

ViroMag CRISPR提高CRISPR/Cas9病毒的转导效率

来源：https://www.amyjet.com/products/OZB-RMC70500.shtml