艾美捷PolyMag CRISPR试剂盒，高转染效率无毒性

艾美捷PolyMag CRISPR试剂盒#PNC40200高转染效率无毒性，灵敏性高、特异性强，用途范围广，具体特色如下：

• 即使在原代细胞和难以转染的细胞上也能高效递送 CRISPR/Cas9 系统• 非常适合共转染 pDNA/pDNA、pDNA/gRNA、pDNA/mRNA

• 针对含有 Cas9 的大质粒 DNA 进行了优化，并引导 RNA

• 低细胞毒性 - 能够以更少的细胞开始实验

PolyMag CRISPR kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). This reagent is based on our proprietary Magnetofection™ technology. The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For your gene editing experiments, this reagent provides high transfection efficiency without toxicity.

Benefits:

•    High efficiency delivery of CRISPR/Cas9 system even on primary and hard to transfect cells

•    Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA

•    Optimized for large plasmid DNA containing Cas9, and guide RNA

•    Low cell toxicity - enable to start your experiment with less cell

用于CRISPR Cas9实验的其他产品：

ViroMag CRISPR提高CRISPR/Cas9病毒的转导效率

RmesFect CRISPR 用于 mRNA 转染

ProDeliverIN CRISPR用于 Cas9 蛋白递送

储存： +4°C

运输条件：室温

Figure1 : PolyMag CRISPR co-transfection efficiency for CRISPR-Cas9 experiments. NIH-3t3, HeLa and MDCK cells were plated in 24-well plates in complete medium at the optimum density of 50-70% confluence at the time of transfection. Cells were transfected with a GFP-Cas9 plasmid and a RFP pDNA and then incubated overnight at 37oC. GFP and RFP expression was monitored 24h after transfection by fluorescence microscopy.

来源：https://www.amyjet.com/products/OZB-PNC40200.shtml