Wheat Germ Agglutinin, AF488 Labeled小麦胚芽凝集素，AF488标记：，享受年度低价

AAT Bioquest-XFD488化学结构与Alexa Fluor®488相同（Alexa Fluor™是ThermoFisher的商标）。麦胚凝集素（WGA）是一种与N-乙酰-D-葡糖胺和唾液酸结合的凝集素。由于其生物学应用，它是研究最多、最有用的凝集素之一。由于WGA与糖复合物结合，其衍生物和偶联物被广泛用于标记细胞膜和纤维化瘢痕组织，用于荧光成像和分析。WGA的碳水化合物结合特异性针对β-1,4-GlcNAc连接的残基序列，即壳聚糖。每个单体包含两个相同的、不相互作用的结合位点，它们与3或4个β-1,4-GlcNAc单元互补。在所研究的单糖中，只有GlcNAc与WGA结合。ManNAc不结合，GalNAc只弱结合。WGA的XFD488共轭物相当于WGA的Alexa Fluor®488共轭物。它表现出明亮的绿色荧光。XFD488 WGA缀合物与唾液酸和N-乙酰氨基葡萄糖残基结合。

艾美捷Wheat Germ Agglutinin, AF488 Labeled小麦胚芽凝集素，AF488标记：

货号：AAT-25500

修正因子（260纳米）：0.30

修正因子（280纳米）：0.11

消光系数（厘米^-1 摩尔^-1）：71000

激发（纳米）：499

发射（纳米）：520

量子产率：0.921

预期用途：仅限研究使用（RUO）

储存：冷冻（< -15°C）；尽量减少光照

荧光显微镜

激发 FITC滤光片组

发射 FITC滤光片组

推荐板 黑色壁，透明底部

图：活HeLa细胞用5µg/mL标记的小麦胚凝集素XFD488染色30分钟，然后用Hoechst 33342（目录号17535）染色。使用FITC和DAPI滤光片组通过荧光显微镜获取图像。

Wheat Germ Agglutinin, AF488 Labeled小麦胚芽凝集素，AF488标记文献参考：

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.

Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida

Journal: Plant physiology and biochemistry : PPB (2020): 420-427

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.

Authors: Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava

Journal: Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.

Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J

Journal: mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.

Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R

Journal: Journal of food science (2020): 808-815

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.

Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu

Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572